Gel electrophoresis! Ran a gel today in lab, and it was super fun! *Note, the top row of wells is mine, I had no hand in the bottom row!
*Second note, it feels pretty cool when yours is the only one is the class that actually banded correctly (other classmates gels had a solid band as if the reaction enzyme did not cut the DNA)
Measuring #transpiration , and therefore #stomatal#aperture , is simple with a #Potometer . A potometer just a tube connected to a calibrated #micropipette and then connected to a leaf in order to measure the movement of water from the calibrated pipette into the leaf. Recoding this change in water level at set time intervals gives a rate of water loss from the system, which is the #Respiration , #Photo -respiration, and #mitochondrial respiration of the leaf. Once you have the rate, you only need the air temperature and relative humidity to determine the stomatal conductance for the leaf. #Stomata#conductance is a measurement of how easily water passes through the stomata, and is a good approximation of #stomatal#aperture .
This is very small, hence the images have been enlarged to enhance the visibility. Here is an abridged demonstration of how to pipette enzymes, and other reagents, at very small volumes. Reagents are mixtures or substances used in chemical a analysis, or chemical analyses, and other reactions.
This is 1 microliter (µl), which is only 0.001 milliliters (mL). When working with such tiny volumes, holding it at the level of your eyes is crucial, but maintain a safe distance from your eyes, face, and other exposed skin. Many enzymes are transparent, thus adequate lighting is significant in these procedures.
It is ineffective to just dispel the small volumes into the tubes, without touching the sides inside of the tube or dispelling it in liquid, due to the very light weight of the reagents, or other substances or mixtures being used, because of Earth's gravitational functionality.