🔬🕳 Plaque Purification ✨✨
When we want to make new viruses that carry our mutation, after cloning we need to plaque purify. What we are doing is making sure that the stock of virus we make is coming from a single virion. This will insure that our viral stock is clonal, or all genetically identical. This is critical because we want to know in future experiments that our mutation is present in all of the virus and that any result we get that is different from another virus can be tied to that mutation we made. •
How do we do it? First we reconstitute the virus. This is done by getting our plasmid, with all the genes for a virus on it, in to our cells. The cells will replicate the DNA and make the viral proteins. The virus can then form completely. From this point on we have actual virus that will replicate as normal. Next we take the virus and dilute is several times. This serial dilution is plated on cells that the virus can infect. We keep an eye 👁 on the plates looking for plaques. Plaques are small clusters of infected cells. These small clusters all start with 1 infected cell. Just what we want! Now comes the hard part, we have to pick just those cells. **see picture** We put a microscope in the tissue culture hood. Find the plaque again and then carefully scrape the plaque with a pipette tip. Once the cells have been loosened we suck them up and put them on fresh, un-infected cells. We let all the cells in that dish get infected, make lots of virus in the process and repeat! We do this process 3 times to make sure the virus is really clonal. •
Sometimes science can be a real pain, not to mention motion sickness inducing, but it really is amazing. From working with DNA at the bench, to now having a clonal viral stock. •
#science#techniques#protocol#stem#scicomm#research#microscope #🔬 #womeninstem#womeninscience#virus#biology#virology#cells#cellculture#tissueculture#lab#experiment#cloning#plaque#purification#clonal
endermologie®, τεχνολογία #madeinFrance που διεγείρει με ήπιο τρόπο το δέρμα ώστε να επανενεργοποιήσει τη κυτταρική διέγερση.
endermologie®, a #madeinFrance technology that gently stimulates the skin to reactivate dormant cellular activity.
Plant and animal cells with my Y7's this week has been a hoot! Always great to see their faces when they look at their own cells under the microscope! 🔬🐵🌱 #teachersfollowteachers#teachersofinstagram#scienceteacher#secondaryteacher#cells#biology#ks3science
Back from my radio silence... been struggling a lot lately with workload and work-life balance but think I'm finally getting back on track!
Hopefully you will be subjected to more posts over the coming weeks as I use this page to keep my focus on the positives! 😊
Memory, thoughts, experiences & health. How are these connected in important ways? I came across this amazing article and wanted to share a part of it with you. ♥️
Cellular Memory Healing: How to Clear Limiting Beliefs and Emotional Wounds at the Cellular Level, by Dr. Alexander Lloyd
“Over the last 50 years and especially the last 15, experts have verified that the source of your symptoms of pain and anxiety is usually not located in your body or even in your environment. The source is located in the unseen issues of your unconscious and subconscious mind, or what science calls “cellular memory...🧠✨
So what exactly do we mean when we say “cellular memories”? Really, we are simply talking about your memories. Researchers began adding the word cellular because we used to believe that all memories were stored in the brain—until, over many years with lots of patients, surgeons removed every part of the brain and found the memories were always still there. The experiences of organ transplant recipients also support this idea. 🧠
Now we know that memories are stored in cells all over the body, but they’re still just what we would call our “memories,” which is how I’ll refer to them throughout the rest of this. Also, out of the various terms writers and researchers have used for cellular memory, I’ve come to prefer the term that King Solomon used, particularly since he was the earliest source I found for this concept: the issues of the heart. But to distinguish this concept from our cardiovascular heart, I call it the “spiritual heart.” 🙏♥️...
So when I refer to the spiritual heart throughout... you can just as easily substitute cellular memory or the subconscious or unconscious mind...
Full article on-line.
Give the gift of Health and Wellness this Christmas!
These are my 4 favourite life giving, health complimenting products I use. I have had life changing experiences from these products. I am happy to chat to anyone who is interested in learning more about the things you can consume to rejuvenate the magnificent body you live in. Please look after your body as its the only place you have to live #christmas#health#cells#redoxmolecules#redoxsignalingmolecules#health#wealth#wellness
Hey there!!!! I have my lemon water and probiotic to get my day started. Lemon water helps with your energy and mood as well as rejuvenate the skin and heals the body. My probiotic has over 50 billion cells that helps with gut health. How are you making the step to help yourself live a happier healthier life?? #detoxwater#probiotic#cells#life#health#minerals#vagina
🌱MẶT NẠ TẾ BÀO GỐC NHAU THAI từ Nhật Bản🌷
Được chiếc xuất từ những thành phần tinh tuý, những dưỡng chất cần thiết nhất cho da như: collagen protein, HA, nhau thai ngựa, tế bào trứng cá hồi, nhau thai người... 🌷Rwine Beauty tự hào mang đến một loại nạ với đầy đủ dinh dưỡng nhất, an toàn nhất, hiệu quả nhất.. ✅ Stem cells mask không những giúp đẹp da, nuôi dưỡng da từ bên trong bởi hàm lượng collagen protein cao mà còn giúp phục hồi da, dịu da, nâng cơ, chống lão hoá, giúp cân bằng độ ẩm, cấp nước và tái sinh cho da thiếu sức sống.... ✅ Stem cell mask giúp ngăn chặn lão hoá, trẻ hoá và giúp da hồng hào, căng bóng mịn. ✅ Stem cell mask không những giúp tái sinh da mõng yếu mà còn giúp da trắng sáng và non da, giúp nâng tone da. ✅ Stem cells mask rất phù hợp cho da sau phi kim, lăn kim, lazer... giúp dịu da giúp da giảm đỏ, giảm sưng, hạn chế cũng như ngăn chặn sắc tố melamin gây sạm và tối màu da. Là một loại mặt nạ không thể thiếu trong liệu trình trị liệu thẩm mỹ của spa, thẩm mỹ viện.. ✳️HDSD: 🌷Massage và làm sạch da mặt trong 3-5 phút bằng ủ nhau thai
🌷Rửa sạch mặt và đắp mặt nạ #Stem#cells trong 30-40 phút
🌷Rửa sạch da và cảm nhận một làn da trắng sáng mịn
🌷Sử dụng các bước chăm sóc da hàng ngày sau khi đắp mặt nạ Stem cells😘 ✳️Có thể sử dụng hàng ngày để phục hồi nhanh cho những loại da mỏng đỏ yếu, da cháy nắng...
In the middle of the month, our focus is still on the feeling of gratitude. One of the recommended oil we are studying this week is Rosemary.
Let's look closer at the healing properties of this oil. 👀
There are six best health uses for Rosemary Essential Oil (including Fighting Cancer!) 1. Decreases Cortisol Levels.
Use your own diffuser (three or four drops) or apply one or two drops on your hands to inhale.
Five minutes of breathing in rosemary helps to decrease salivary cortisol levels. Cortisol, released in response to stress by the adrenal glands,is sometimes referred to as the “stress hormone.” Japanese researchers observed that the antioxidants in rosemary essential oil enhanced the body’s free radical scavenging ability.
2. Inhibits Deadly Bacteria.
Rosemary has long been used to improve skin by fighting bacteria and by cell renewal. Rosemary was able to significantly inhibit Pseudomonas aeruginosa and had strong antimicrobial activity against Staphylococcus aureus. Theses bacteries can cuase a range of illnesses including life-threatening diseases such as pneumonia.
3. Reduces Inflammation.
Rosemary essential oil has anti-inflammatory properties and could be considered a possible remedy for colitis, an inflammatory bowel disease.
4. Fights Cancer affecting Liver, Colon, Breast, Skin and Lung.
5. Aids Hair Regrowth.
Researchers, comparing it with minoxidil (a commonly used drug for combating hair loss) found rosemary essential oil to be just as good as minoxidil (after 6 months of use). 6. Assists Brain Health.
Rosemary has the ability to help overcome mental fatigue, improving mental clarity and focus. More than that, it may be helpful in the prevention of Alzheimer’s Disease.
Finally this oil can be taken internally, dilute one drop in 4 fl. oz. of liquid.
Awaken your mind and bring warmth to your heart with Rosemary.
We clean everything, but the inside of our bodies. Detoxing at cellular level is very important. No only do you feel the difference, after couple session you see the difference.
The ionic bath works by charging a bath, generally a foot bath, with ions that enter your body through the feet to counteract the excess ions in your cells, with the idea being to restore a healthy electrical balance at the cellular level. Each cell in your body carries its own charge, and if that charge is not in a healthy range, your well-being may suffer. Another goal of the ionic bath is to provide a medium through which impurities, heavy metals and toxins can travel from the body into the ionized water through osmosis.
Testimony from clients,
Clearer skin, cyst removed, liver function improvement, heavy metal reduction, menstrual flow decrease, weight loss, clarity, inflammation reduced, asthma relief, improved breathing, mucus reduction, headaches, skin tone, stiffness, parasites removal, sex drive stimulated, gallbladder function improvement, joint pain, tension , this list goes on and on...
What are you waiting on? Cleanse today!!
Bjutiful Soul Detox Mobile Spa
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